Journal:

Article Title: Efficient Transfer of the Pheromone-Independent Enterococcus faecium Plasmid pMG1 (Gm r ) (65.1 Kilobases) to Enterococcus Strains during Broth Mating

doi:

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: Antibiotics were used at the following concentrations: ampicillin, 100 μg/ml; erythromycin, 12.5 μg/ml; chloramphenicol, 20 μg/ml; fusidic acid, 25 μg/ml; rifampin, 25 μg/ml; tetracycline, 10 μg/ml; streptomycin, 500 μg/ml; kanamycin, 500 μg/ml; gentamicin, 500 μg/ml. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Genotype or phenotype Description and source or reference Strains E. faecalis FA2-2 rif fus Derivative of JH2; 8 E. faecalis JH2SS str spc Derivative of JH2; 42 E. faecium KTRF rif fus Derivative of plasmid-free E. faecium KT; this study E. faecium KTSS str spc Derivative of plasmid-free E. faecium KT; this study E. hirae 9790RF rif fus Derivative of E. hirae ATCC 9790 E. hirae 9790SS str spc Derivative of E. hirae ATCC 9790 E. coli DH1 F − recA1 endA1 gryA96 thi-1 relA1 hsdR17 supE44 36 Plasmids pMG1 Gm r 65.1-kb conjugative plasmid from E. faecium strain; this study pAD1 hly/bac uvr 59.6-kb pheromone-responsive conjugative plasmid from DS16; 8 pAM714 hly/bac erm pAD1::Tn 917 , wild-type transfer; 23 pPD1 bac 59-kb pheromone-responsive conjugative plasmid from E. faecalis 39-5; 49 pAM373 tet 36-kb pheromone-responsive conjugative plasmid; 7 pAMβ1 erm 26.5-kb broad-host-range conjugative plasmid from DS5; 30 pIP501 erm cat 39.2-kb broad-host-range conjugative plasmid; 13 pAM120 amp tet pBR322-derived vector pGL101 carrying pAD1 Eco R1 fragment F::Tn 916 ; 18 pMG326 amp lacZ E. coli vector pMW119 ( amp lacZ ) (Nippon Gene Co., Ltd.) carrying a part (11.5 kb) of pPD1 Eco RI fragment A; 37 pAM401 cat tet E. coli-E. faecalis shuttle vector; 48 Open in a separate window Bacterial strains and plasmids

Techniques: Plasmid Preparation